S-Nitrosylation Signaling in Cell Biology

Acivicin reverses the effects of GSNO. A. GSNO-induced HIF-1 DNA binding activity is reversed by acivicin. Nuclear extracts made from bovine pulmonary artery endothelial cells were treated with 100 μM GSNO in the absence (–) or presence (+) or 100 μM acivicin for 4 h. HIF-1 DNA binding activity was determined by electrophoretic mobility shift assay using 3 μg nuclear protein and 1.5 fmol of a 30-bp oligonucleotide containing the HIF-1 DNA binding site. Reprinted with permission from (41) B. GSNO induction of CFTR maturation is inhibited by acivicin and reversed by DTT. Western blot anlysis was performed on 100 μg of wholecell extracts made from CFPAC-1 cells treated with 1 μM GSNO in the presence of 100 μM acivicin for 4 h or 200 μM DTT drug in the last 30 min of the 4 h incubation time. Reprinted with permission (42) C. The GSNO induced-increase in minute ventilation (V_E) is blocked by acivicin. V_E increases stimulated by microinjection of 10 nmol GSNO were abolished after pretreatment with the gamma glutamyl transpeptidase inhibitor acivicin (7.5 nmol). CGSNO (10 nmol) stimulated V_E increased were not modified by acivicin. * indicates statistically significant differences compared to controls. Reprinted with permission (17).