ORIGINAL ARTICLE |
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Year : 2014 | Volume
: 2
| Issue : 3 | Page : 71-75 |
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In vitro evaluation of cytotoxic effects of luting resin cements
Meral Arslan Malkoc1, Muhammet Yalcin2, Ismail Hakki Uzun1, Ali Riza Tuncdemir3
1 Department of Prosthetic Dentistry, Faculty of Dentistry, Inonu University, Malatya, Turkey 2 Department of Restorative Dentistry, Faculty of Dentistry, Inonu University, Malatya, Turkey 3 Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkey
Correspondence Address:
Meral Arslan Malkoc Department of Prosthetic Dentistry, Faculty of Dentistry, Inonu University, Malatya 44280 Turkey
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DOI: 10.4103/2347-4610.140513
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Purpose: This in vitro study evaluated the cytotoxicity of resin cements with L929 fibroblasts. Materials and Methods: The resin cements, Super-Bond C&B, RelyX ARC, Clearfil Esthetic, specimens were prepared and extracted. The L929 cells were plated (25,000 cells/mL) and maintained. The medium was replaced with the resin extracts. Twelve specimens were used for each group. Cell viability was evaluated by 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) tests. The data were analyzed by one-way analysis of variance and Tukey honest significant difference tests. Results: Clearfil Esthetic had significantly higher decreasing effect on cell survival and growth (55.64 ± 8.26%), from the other resin cements and the control (P < 001). There are no differences between the survival rates of Super-Bond C&B (72.75 ± 12.33%) and RelyX ARC (62.63 ± 10.98%) (P > 05); however, each of them was cytotoxic when they compared with the control (P < 001). Conclusion: Differential toxic effects of resin-based cement on the pulp cells should be considered during the selection. |
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