Genome-Wide Linkage and Association Scans for Quantitative Trait Loci of Serum Lactate Dehydrogenase—The Framingham Heart Study

Lin, Jing-Ping; Zheng, Gang; Joo, Jungnam; Cupples, L. Adrienne

doi:10.4061/2010/905237

Genome-Wide Linkage and Association Scans for Quantitative Trait Loci of Serum Lactate Dehydrogenase—The Framingham Heart Study

Jing-Ping Lin linj{at}nhlbi.nih.gov¹
Gang Zheng zhengg{at}nhlbi.nih.gov¹
Jungnam Joo jooj{at}nhlbi.nih.gov¹
L. Adrienne Cupples adrienne{at}bu.edu²

¹Office of Biostatistics Research, Division of Cardiovascular Sciences, National Heart, Lung and Blood Institute, NIH 6701 Rockledge Dr. Suite 9196, Bethesda, MD 20892-7913, USA
²Department of Biostatistics, Boston University School of Public Health, MA 02118, USA

Abstract

Serum lactate dehydrogenase (LDH) is used in diagnosing many diseases and is significantly determined by genetic factors. Three genes coding for LDH isoenzymes were mapped to chromosome 11q15 and 12p12. We used 330 Framingham Heart Study largest families for microsatellite linkage scan and 100K SNPs association scan to determine quantitative trait loci of LDH level. We estimated the heritability at 41%. Our genome-wide linkage analysis yielded several chromosomal regions, other than 11q and 12p, with LOD scores between 1 and 2.5. None of the 100K SNPs with a P-value $\text{[math]}$ in our genome-wide association study was close to the chromosomal regions where the LDH genes reside. Our study demonstrated a strong genetic effect on the variation of LDH levels. There may not be a single gene with a large effect, instead may be several genes with small effects in controlling the variation of serum LDH. Those genes may be located on chromosomal regions that differ from where the genes encoding LDH isoenzymes reside.