ROLE of MITOCHONDRIA in TOXIC OXIDATIVE STRESS

Effect of dietary TA supplementation on (A) T levels, on (B) susceptibility to lipid peroxidation in liver homogenates and inner mitochondrial membranes (IMM), or on (C) Fe²⁺-induced cell death. Male Sprague Dawley rats were given the diets that included TA supplements (50, 250, or 1400 IU/kg diet) for seven days. Liver homogenates (Homog) and IMMs or hepatocytes were isolated and examined by methods described elsewhere (21). Fe²⁺ -induced cell death was measured after two hours. Leakage of lactate dehydrogenase (LDH) from cells used as a measure of necrotic cell death. Data are the mean ± SE of three separate animals per treatments. *p<0.05, as compared with 50 IU/kg diet group. Figure adapted from (11).