- Institution: Stanford Univ Med Ctr Lane Med Lib/Periodical Dept/Rm L109
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ROLE of MITOCHONDRIA in TOXIC OXIDATIVE STRESS
Table 1.
Protective Effect of Tocopheryl Succinate in Hepatocytes Exposed to a Variety of Toxic Oxidative Insults
| % Cell Death | ||
|---|---|---|
| Toxic Insults | Medium Without TS | Medium With TS |
| Freshly isolated rat hepatocyte suspensions were pretreated with 25 μM vitamin E succinate (TS) or vehicle (ethanol) for fifteen minutes prior to treatment with a toxic insult. Cell death (%LDH leakage) was measured hourly in each suspension for up to six hours. Data points are the average of results obtained from at least three separate hepatocyte preparations and are representative of two separate experiments. | ||
| Control (Vehicle Only) | 21% | 21% |
| Adriamycin/BCNU (100/50 μM) | 62% | 28% |
| Ionophore A23187 (5 μM) | 73% | 24% |
| 95% Oxygen (Hyperoxia) | 57% | 23% |
| Ferrous Ammonium Sulfate (0.75 mM) | 59% | 29% |
| Acrolein (150 μM) | 64% | 26% |
| Ethyl Methanesulfonate (EMS) (35 mM) | 68% | 25% |
| Rotenone/DEM (100 μM/1 mM) | 69% | 28% |
