- Institution: Stanford Univ Med Ctr Lane Med Lib/Periodical Dept/Rm L109
- Sign In as Member / Individual
The Choline Transporter Resurfaces: New Roles for Synaptic Vesicles?
Table 1.
Rapid CHT Regulation: A Conserved Relationship with Cholinergic Activation
| Tissue Preparation | Species | Treatment | Effect | References |
|---|---|---|---|---|
| Preganglionic sympathetic and parasympathetic neurons; forebrain cholinergic neurons | Cat, chicken, rat | Electrical stimulation | Parallel modulation of choline uptake and ACh synthesis | (10, 55, 59–61, 163) |
| Various forebrain preparations | Rat | In vivo pharmacological modulation of ACh release | CHT Vmax and Bmax for HC-3 binding regulated in parallel with ACh release | (56, 57, 63, 71, 75) |
| Various CNS preparations | Rat, mouse, horseshoe crab | Depolarization of sample with high [K+] buffer | Increased CHT Vmax and Bmax for HC-3 binding | (64, 65, 76, 138, 164) |
| Striatal plasma membrane enriched preparation | Rat | Treatment of membranes with PLA2 or arachidonic acid | Increased Bmax for HC-3 binding | (132, 136, 137) |
| Hippocampal synaptosomes | Rat | Preincubation with serine– threonine phosphatase inhibitors | Decreased CHT Vmax | (101) |
| Electric organ synaptosomes | Torpedo | Preincubation with cyclosporine A | Deceased choline uptake | (88) |
| Striatal membranes | Rat | Preincubation of membranes ± Mg2+ and ATP | Altered affinity for HC-3 binding | (38, 143) |
| Brain synaptosomes | Locust | Preincubation with cAMP analogues | Increased HC-3 binding | (165) |
| Brain synaptosomes | Rat | Disruption of microtubules with colchicine | Decreased choline uptake | (130) |
| Hippocampal synaptosomes and membrane preparations | Mouse | Transgenic mice over- expressing AChE | Increased HC-3 binding and choline uptake | (150, 151) |
| Hippocampal and cortical synaptosomes | Rat and mouse | Behavioral training (Including radial maze and T maze) | Increased choline uptake | (67–71) |
